Methods

  • Full spectrum of DNA-, RNA-, and protein methods
  • Mammalian cell culture (cell lines and primary cells)
  • Gene-vector technology and somatic gene therapy
  • RNA interference (RNAi)-technology
  • (Realtime-) Fluorescence analyses (indirect IF, GFP etc.)
  • (Realtime-) PCR
  • Expression and purification of recombinant proteins
  • HIV and HSV infection experiments in tissue culture
  • Humanized mouse models for HIV-infection

    Recent Publications

    Chemnitz J, Pieper D, Stich L, Schumacher U, Balabanov S, Spohn M, Grundhoff A, Steinkasserer A, Hauber J, Zinser E (2019). The acidic protein rich in leucines Anp32b is an immunomodulator of inflammation in mice. Sci Rep. 2019 Mar 19;9(1):4853.

    Bialek JK, Walther T, Hauber J, Lange UC (2018). CRISPR-Cas9-based Genome Engineering to Generate Jurkat Reporter Models for HIV-1 Infection with Selected Proviral Integration Sites. J Vis Exp. 2018 Nov 14;(141).

    Brunnemann AK, Hoffmann A, Deinhardt-Emmer S, Nagel CH, Rose R, Fickenscher H, Sauerbrei A, Krumbholz A. (2018). Relevance of non-synonymous thymidine kinase mutations for antiviral resistance of recombinant herpes simplex virus type 2 strains. Antiviral Res. 2018 Apr;152:53-57.