Methods

    • tissue culture techniques
    • transfection/infection of mamalian cells
    • transformation reporter gene assays in primary mamalian cells
    • RNA interference (RNAi) technology
    • fluorescence microscopy
    • multicolor flow cytomerty
    • protein/protein nteractions
    • DNA/protein interactions (chromatin immunoprecipitation, ChIP)
    • 2D-gelelektrophoresis/ MALDI-ToF mass spectroscopy
    • expression and purification of recombinant proteins
    • generation and characterization of novel antibodies
    • isolation, identification, high-titer production of viruses
    • phentotypic characterization
    • generation of recombinant  adenoviral vectors/oncolytic viruses
    • quantitative real time PCR
    • FISH (Fluoreszenz in situ hybridisation)
    • generation and transduction of recombinant lentiviral particles
    • humanized mouse models of infection/bone marrow chimeric mice

        Recent Publications

        Czech-Sioli M, Siebels S, Radau S, Zahedi RP, Schmidt C, Dobner T, Grundhoff A, Fischer N (2020). The Ubiquitin-Specific Protease Usp7, a Novel Merkel Cell Polyomavirus Large T-Antigen Interaction Partner, Modulates Viral DNA Replication. J Virol. 2020 Feb 14;94(5).

        Hofmann-Sieber H, Gonzalez G, Spohn M, Dobner T, Kajon AE (2020). GenOMIC AND Phylogenetic analysis of TWO Guinea Pig Adenovirus STRAINS RECOVERED from archival LUNG tissue. Virus Res. 2020 Apr 17:197965.

        Stubbe M, Mai J, Paulus C, Stubbe HC, Berscheminski J, Karimi M, Hofmann S, Weber E, Hadian K, Hay R, Groitl P, Nevels M, Dobner T, Schreiner S (2020). Viral DNA Binding Protein SUMOylation Promotes PML Nuclear Body Localization Next to Viral Replication Centers. mBio. 2020 Mar 17;11(2).