Methods

    • tissue culture techniques
    • transfection/infection of mamalian cells
    • transformation reporter gene assays in primary mamalian cells
    • RNA interference (RNAi) technology
    • fluorescence microscopy
    • multicolor flow cytomerty
    • protein/protein nteractions
    • DNA/protein interactions (chromatin immunoprecipitation, ChIP)
    • 2D-gelelektrophoresis/ MALDI-ToF mass spectroscopy
    • expression and purification of recombinant proteins
    • generation and characterization of novel antibodies
    • isolation, identification, high-titer production of viruses
    • phentotypic characterization
    • generation of recombinant  adenoviral vectors/oncolytic viruses
    • quantitative real time PCR
    • FISH (Fluoreszenz in situ hybridisation)
    • generation and transduction of recombinant lentiviral particles
    • humanized mouse models of infection/bone marrow chimeric mice

        Recent Publications

        Dhingra A, Hage E, Ganzenmueller T, Böttcher S, Hofmann J, Hamprecht K, Obermeier P, Rath B, Hausmann F, Dobner T, Heim A (2019). Molecular Evolution of Human Adenovirus (HAdV) Species C. Sci Rep. 2019 Jan 31;9(1):1039.

        Tejera B, López RE, Hidalgo P, Cárdenas R, Ballesteros G, Rivillas L, French L, Amero C, Pastor N, Santiago Á, Groitl P, Dobner T, Gonzalez RA (2019). The human adenovirus type 5 E1B 55kDa protein interacts with RNA promoting timely DNA replication and viral late mRNA metabolism. PLoS One. 2019 Apr 3;14(4):e0214882.

        Hagkarim NC, Ryan EL, Byrd PJ, Hollingworth R, Shimwell NJ, Agathanggelou A, Vavasseur M, Kolbe V, Speiseder T, Dobner T, Stewart GS, Grand RJ. (2018). Degradation of a novel DNA damage response protein, tankyrase 1 binding protein 1 (Tab182), following adenovirus infection. J Virol. 2018 May 29;92(12).