Methods

    • tissue culture techniques
    • transfection/infection of mamalian cells
    • transformation reporter gene assays in primary mamalian cells
    • RNA interference (RNAi) technology
    • fluorescence microscopy
    • multicolor flow cytomerty
    • protein/protein nteractions
    • DNA/protein interactions (chromatin immunoprecipitation, ChIP)
    • 2D-gelelektrophoresis/ MALDI-ToF mass spectroscopy
    • expression and purification of recombinant proteins
    • generation and characterization of novel antibodies
    • isolation, identification, high-titer production of viruses
    • phentotypic characterization
    • generation of recombinant  adenoviral vectors/oncolytic viruses
    • quantitative real time PCR
    • FISH (Fluoreszenz in situ hybridisation)
    • generation and transduction of recombinant lentiviral particles
    • humanized mouse models of infection/bone marrow chimeric mice

        Recent Publications

        Czech-Sioli M, Siebels S, Radau S, Zahedi RP, Schmidt C, Dobner T, Grundhoff A, Fischer N (2020). The Ubiquitin-Specific Protease Usp7, a Novel Merkel Cell Polyomavirus Large T-Antigen Interaction Partner, Modulates Viral DNA Replication. J Virol. 2020 Feb 14;94(5):e01638-19.

        Hofmann-Sieber H, Gonzalez G, Spohn M, Dobner T, Kajon AE (2020). Genomic and phylogenetic analysis of two guinea pig adenovirus strains recovered from archival lung tissue. Virus Res. 2020 Aug;285:197965.

        Pfitzner S, Hofmann-Sieber H, Bosse JB, Franken LE, Grünewald K, Dobner T (2020). Fluorescent Protein Tagging of Adenoviral Proteins pV and pIX Reveals 'Late Virion Accumulation Compartment'. PLoS Pathog. 2020 Jun 25;16(6):e1008588.