Methods

    • Mammalian cell culture (primary cells and cell lines)
    • Tissue processing (liver, gut, spleen)
    • Tissue-culture techniques under S2 and S3** conditions
    • Single-cell cloning
    • HIV-1 in vitro infection models
    • HIV-1 latency models
    • Assessment of NK cell function
    • Jurkat-Reporter assay (KIR/HLA interactions)
    • HLA stabilization assay
    • Generation of HLA Class I tetramers
    • Multiparameter flow cytometry (20 parameters)
    • Fluorescent-activated cell sorting (S3**, 4-way, 96-well, single cell)
    • RNA Flow cytometry (Prime Flow)
    • Chipcytometry
    • Seahorse Assay (Metabolism)
    • Multiplex immunoassays for Luminex
    • Molecular-biological and biochemical methods (DNA/RNA/protein)
    • RNA interference (siRNA)
    • Genome-wide CRISPR knock-out screen (GeCKO)/CRISPR/Cas9
    • Single-cell genomics/ single-cell transcriptome analysis (Fluidigm)
    • Organoid techniques including  parenchym-immune cell co-cultures in organoid systems
    • Viral infection of primary cells in organoids

      Publications (Selection)

      Claiborne DT, Scully EP, Palmer CD, Prince JL, Macharia GN, Kopycinski J, Michelo CM, Wiener HW, Parker R, Nganou-Makamdop K, Douek D, Altfeld M, Gilmour J, Price MA, Tang J, Kilembe W, Allen SA, Hunter E (2019). Protective HLA alleles are associated with reduced LPS levels in acute HIV infection with implications for immune activation and pathogenesis. PLoS Pathog. 2019 Aug 26;15(8):e1007981.

      Langeneckert AE, Lunemann S, Martrus G, Salzberger W, Hess LU, Ziegler AE, Poch T, Ravichandran G, Matschl U, Bosse JB, Tiegs G, Fischer L, Koch M, Herkel J, Oldhafer KJ, Schramm C, Altfeld M (2019). CCL21-expression and accumulation of CCR7+ NK cells in livers of patients with primary sclerosing cholangitis. Eur J Immunol. 2019 May;49(5):758-769.

      Lotter H, Altfeld M (2019). Sex differences in immunity. Semin Immunopathol. 2019 Mar;41(2):133-135.