Methods

    • Mammalian cell culture (primary cells and cell lines)
    • Tissue processing (liver, gut, spleen)
    • Tissue-culture techniques under S2 and S3** conditions
    • Single-cell cloning
    • HIV-1 in vitro infection models
    • HIV-1 latency models
    • Assessment of NK cell function
    • Jurkat-Reporter assay (KIR/HLA interactions)
    • HLA stabilization assay
    • Generation of HLA Class I tetramers
    • Multiparameter flow cytometry (20 parameters)
    • Fluorescent-activated cell sorting (S3**, 4-way, 96-well, single cell)
    • RNA Flow cytometry (Prime Flow)
    • Chipcytometry
    • Seahorse Assay (Metabolism)
    • Multiplex immunoassays for Luminex
    • Molecular-biological and biochemical methods (DNA/RNA/protein)
    • RNA interference (siRNA)
    • Genome-wide CRISPR knock-out screen (GeCKO)/CRISPR/Cas9
    • Single-cell genomics/ single-cell transcriptome analysis (Fluidigm)
    • Organoid techniques including  parenchym-immune cell co-cultures in organoid systems
    • Viral infection of primary cells in organoids

      Publications (Selection)

      Adland E, Millar J, Bengu N, Muenchhoff M, Fillis R, Sprenger K, Ntlantsana V, Roider J, Vieira V, Govender K, Adamson J, Nxele N, Ochsenbauer C, Kappes J, Mori L, van Lobenstein J, Graza Y, Chinniah K, (...), Altfeld M, Goulder P (2020). Sex-specific innate immune selection of HIV-1 in utero is associated with increased female susceptibility to infection. Nat Commun. 2020 Apr 14;11(1):1767.

      Hess LU, Martrus G, Ziegler AE, Langeneckert AE, Salzberger W, Goebels H, Sagebiel AF, Hagen SH, Poch T, Ravichandran G, Koch M, Schramm C, Oldhafer KJ, Fischer L, Tiegs G, Richert L, Bunders MJ, Lunemann S, Altfeld M (2020). The Transcription Factor Promyelocytic Leukemia Zinc Finger Protein Is Associated With Expression of Liver-Homing Receptors on Human Blood CD56bright Natural Killer Cells. Hepatol Commun. 2020 Jan 6;4(3):409-424.

      Kunzmann LK, Schoknecht T, Poch T, Henze L, Stein S, Kriz M, Grewe I, Preti M, Hartl J, Pannicke N, Peiseler M, Sebode M, Zenouzi R, (...), Hess LU, Elise Ahrenstorf A, Lunemann S, Martrus G, (...), Schramm C, Schwinge D, Li (2020). Monocytes as potential mediators of pathogen-induced Th17 differentiation in patients with primary sclerosing cholangitis (PSC). Hepatology. 2020 Jan 23.