Range of methods

Electron microscopy methods

Transmission electron microscopy [Philips CM120 with Gatan MSC 794]

Electron tomography [FEI Tecnai G20 Olympus Veleta and FEI Eagle 4k]

Scanning electron microscopy [Philips XL30ESEM with Point Electronic DISS5]

Environmental scanning electron microscopy (ESEM) [Philips XL30ESEM ]

Correlative light and electron microscopy

Light microscopy methods

Time-lapse microscopy [Nikon Biostation IM]

Confocal laser scanning microscopy [Nikon C2 plus, Zeiss LSM510 META FCS]

Total internal reflection fluorescence microscopy (TIRFM) [Nikon Ti TIRF]

Stochastic Optical Reconstruction Microscopy (STORM) [Nikon N- STORM]

High-resolution differential interference contrast microscopy [Nikon Eclipse Ti]

Reflected light differential interference contrast microscopy [Nikon AZ100]

High-resolution external apodized phase contrast [Nikon Eclipse Ti]

Dark -field microscopy [Nikon Eclipse Ti]

High content (confocal) fluorescence microscopy, [BD Pathway 855]

Preparation methods

High-pressure freezing [BalTec HPM010], freeze substitution [Leica AFS]

(Cryo-) ultramicrotomy [UC7/FC7 Leica, Ultracut UCT]

Metal shadowing / freeze etching / replica technique [BalTec BAF060]

Critical point drying [Polaron E3000]

Sputter coating [quorum Q150R ES]

Vibratome [Leica VT1200S]

Micro biopsy technique [self-development]

In situ preparation in cellulose micro capillaries [CarboCell, self- development]

Negative staining

Immunolabeling

Data processing / analysis

Quantitative analysis [ImageJ, NIS-Elements, Imaris]

Deconvolution [ Autodeblur, NIS elements, Zeiss AIM]

3D reconstruction [Imaris, Amira]

Stereoscopy [ImageJ , self-development]

Animations [Cinema4D]

 

Recent Publications

Hernández Durán A, Greco TM, Vollmer B, Cristea IM, Grünewald K, Topf M (2019). Protein interactions and consensus clustering analysis uncover insights into herpesvirus virion structure and function relationships. PLoS Biol. 2019 Jun 14;17(6):e3000316.

Hernández Durán A, Grünewald K, Topf M (2019). Conserved Central Intraviral Protein Interactome of the Herpesviridae Family. mSystems. 2019 Oct 1;4(5).

Langeneckert AE, Lunemann S, Martrus G, Salzberger W, Hess LU, Ziegler AE, Poch T, Ravichandran G, Matschl U, Bosse JB, Tiegs G, Fischer L, Koch M, Herkel J, Oldhafer KJ, Schramm C, Altfeld M (2019). CCL21-expression and accumulation of CCR7+ NK cells in livers of patients with primary sclerosing cholangitis. Eur J Immunol. 2019 May;49(5):758-769.