Range of methods

Electron microscopy methods

Transmission electron microscopy [Philips CM120 with Gatan MSC 794]

Electron tomography [FEI Tecnai G20 Olympus Veleta and FEI Eagle 4k]

Scanning electron microscopy [Philips XL30ESEM with Point Electronic DISS5]

Environmental scanning electron microscopy (ESEM) [Philips XL30ESEM ]

Correlative light and electron microscopy

Light microscopy methods

Time-lapse microscopy [Nikon Biostation IM]

Confocal laser scanning microscopy [Nikon C2 plus, Zeiss LSM510 META FCS]

Total internal reflection fluorescence microscopy (TIRFM) [Nikon Ti TIRF]

Stochastic Optical Reconstruction Microscopy (STORM) [Nikon N- STORM]

High-resolution differential interference contrast microscopy [Nikon Eclipse Ti]

Reflected light differential interference contrast microscopy [Nikon AZ100]

High-resolution external apodized phase contrast [Nikon Eclipse Ti]

Dark -field microscopy [Nikon Eclipse Ti]

High content (confocal) fluorescence microscopy, [BD Pathway 855]

Preparation methods

High-pressure freezing [BalTec HPM010], freeze substitution [Leica AFS]

(Cryo-) ultramicrotomy [UC7/FC7 Leica, Ultracut UCT]

Metal shadowing / freeze etching / replica technique [BalTec BAF060]

Critical point drying [Polaron E3000]

Sputter coating [quorum Q150R ES]

Vibratome [Leica VT1200S]

Micro biopsy technique [self-development]

In situ preparation in cellulose micro capillaries [CarboCell, self- development]

Negative staining

Immunolabeling

Data processing / analysis

Quantitative analysis [ImageJ, NIS-Elements, Imaris]

Deconvolution [ Autodeblur, NIS elements, Zeiss AIM]

3D reconstruction [Imaris, Amira]

Stereoscopy [ImageJ , self-development]

Animations [Cinema4D]

 

Recent Publications

Chorev DS, Tang H, Rouse SL, Bolla JR, von Kügelgen A, Baker LA, Wu D, Gault J, Grünewald K, Bharat TAM, Matthews SJ, Robinson CV (2020). The use of sonicated lipid vesicles for mass spectrometry of membrane protein complexes. Nat Protoc. 2020 May;15(5):1690-1706.

Franken LE, Grünewald K, Boekema EJ, Stuart MCA (2020). A Technical Introduction to Transmission Electron Microscopy for Soft-Matter: Imaging, Possibilities, Choices, and Technical Developments. Small. 2020 Mar 4:e1906198.

Pfitzner S, Hofmann-Sieber H, Bosse JB, Franken LE, Grünewald K, Dobner T (2020). Fluorescent Protein Tagging of Adenoviral Proteins pV and pIX Reveals 'Late Virion Accumulation Compartment'. PLoS Pathog. 2020 Jun 25;16(6):e1008588.